Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling MAGEA1 with ab227664 at 1/100 dilution (1.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purifiedab227664 at 1:20 dilution (7.25 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Formalin-fixed, paraffin-embedded human testis tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.

Anti-MAGEA1 antibody [SP188] (ab227664) at 1/400 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 34 kDa

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling MAGEA1 with ab227664 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.