Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MAGEA1 antibody [EPR4276] (ab109108)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4276] to MAGEA1
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-MAGEA1 antibody [EPR4276]
See all MAGEA1 primary antibodies
Description

Rabbit monoclonal [EPR4276] to MAGEA1
Host species

Rabbit
Specificity

Based on the sequence blast, the immunogen of ab109108 has 85.7% - 92.3% homology with the family members MAGEB3, MAGE2, MAGE3, MAGE6, MAGE9, MAGE10 and MAGE12

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A375, HeLa, Jurkat, HepG2, and MCF7 lysates. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
General notes
This product was previously labelled as MAGE 1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4276
Isotype

IgG
Research areas

Western blot - Anti-MAGEA1 antibody [EPR4276] (ab109108)

All lanes : Anti-MAGEA1 antibody [EPR4276] (ab109108) at 1/1000 dilution

Lane 1 : A375 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 34 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-MAGEA1 antibody [EPR4276] (ab109108)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAGEA1 with ab109108 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only.
Flow Cytometry - Anti-MAGEA1 antibody [EPR4276] (ab109108)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling MAGEA1 with ab109108 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-MAGEA1 antibody [EPR4276] (ab109108)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com