Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸ 1 ml 465 705 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP194] to MAGEA1 - C-terminal
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-MAGEA1 antibody [SP194] - C-terminal
See all MAGEA1 primary antibodies
Description

Rabbit monoclonal [SP194] to MAGEA1 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human HeLa and A431 cell lysates. Human A431 cells. Human testis and skin squamous cell carcinoma tissues. Flow Cyt: SK-OV-3 cells
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as MAGE 1

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP194
Isotype

IgG
Research areas

Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purified ab183309 at 1/20 dilution (8 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Immunohistochemical analysis of Human testis tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Immunohistochemical analysis of Human skin squamous cell carcinoma tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.
Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Flow cytometric analysis of Human A431 cells labeling MAGEA1 using ab183309(1/400) (green) compared to a negative control rabbit IgG (blue)
Western blot - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309) at 1/400 dilution + Human A431 cell lysate

Predicted band size: 34 kDa
Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com