Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ran antibody [EPR10791(B)] (ab155103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10791(B)] to Ran
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ran antibody [EPR10791(B)]
    See all Ran primary antibodies

  • Description

    Rabbit monoclonal [EPR10791(B)] to Ran

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Ran. The exact sequence is proprietary.
    Database link: P62826
    (Peptide available as ab217263)

  • Positive control

    • WB: HepG2, LoVo, Jurkat, HeLa, C6, Raw 264.7, PC-12, and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate; Flow Cyt: HeLa cells; ICC: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Western blot - Anti-Ran antibody [EPR10791(B)] (ab155103)
    All lanes : Anti-Ran antibody [EPR10791(B)] (ab155103) at 1/1000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    We are unsure how to define the extra bands.

  • Immunocytochemistry - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Immunocytochemistry - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Flow Cytometry - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ran with purified ab155103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] (ab155103)
    Immunoprecipitation - Anti-Ran antibody [EPR10791(B)] (ab155103)

    Purified ab155103 at 1/50 dilution (2 µg) immunoprecipitating Ran in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab155103 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155103 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 24 kDa

  • Anti-Ran antibody [EPR10791(B)] (ab155103)
    Anti-Ran antibody [EPR10791(B)] (ab155103)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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