Anti-Ran antibody [8D1H12] (ab233762)
Key features and details
- Mouse monoclonal [8D1H12] to Ran
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Ran antibody [8D1H12]
See all Ran primary antibodies -
Description
Mouse monoclonal [8D1H12] to Ran -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human Ran aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P62826 -
Positive control
- WB: A431, C6, Jurkat, HeLa and COS-7 cell lysate; Recombinant human Ran protein; RAN (AA: 1-216)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human esophageal cancer and rectum cancer tissue. Flow: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8D1H12 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : C6 (Rat glial tumor cell line) cell lysate
Lanes 3 & 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (ab233762)
Paraffin-embedded human rectum cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (ab233762)
Paraffin-embedded human esophageal cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Ran using ab233762 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : RAN (AA: 1-216)-hIgGFc transfected HEK-293 cell lysate
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Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution + Recombinant human Ran protein
Expected MW is 50.4 kDa.