Recombinant human LDL Receptor protein (Active) (ab220582)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: StrepII tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant human LDL Receptor protein (Active) (ab220582)Human LDL R Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220582)Immobilized ab220582 at 10 μg/mL (100 μl/well) can bind Rhesus macaque PCSK9, His Tag with a linear range of 2-15 ng/mL.
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SDS-PAGE - Recombinant human LDL Receptor protein (Active) (ab220582)SDS-PAGE analysis of reduced ab220582 stained overnight with Coomassie Blue.
The protein migrates as 125-140 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
