Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant human LDL Receptor protein (Active) (ab220570)

Key features and details

Expression system: HEK 293 cells
Purity: > 90% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: Functional Studies, SDS-PAGE

Alternative names
- FH
- FHC
- LDL R
- LDL receptor
- LDLCQ2
- Ldlr
- LDLR_HUMAN
- Low Density Lipoprotein Receptor
- Low density lipoprotein receptor class A domain containing protein 3
- Low density lipoprotein receptor familial hypercholesterolemia
- Low-density lipoprotein receptor
see all
Function

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Involvement in disease

Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
Sequence similarities

Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.
Post-translational
modifications

N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Cellular localization

Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
Target information above from: UniProt accession P01130 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)

Immobilized Human PCSK9 (D374Y) (His Tag) at 5µg/mL (100 µL/well) can bind Human LDL R (Fc Tag) with a linear range of 8-320 ng/mL (QC tested).
SDS-PAGE - Recombinant human LDL Receptor protein (Active) (ab220570)

ab220570 on SDS-PAGE under reducing (lane 1) and no-reducing (lane 2) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. As a result of glycosylation, the reduced protein migrates at 155-175 kDa and the non-reduced protein migrates at 310-350 kDa.
Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)

Example of ab220570 bioactivity.

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