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Recombinant human LDL Receptor protein (Active) (ab220570)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Preparation and Storage

  • Alternative names

    • FH
    • FHC
    • LDL R
    • LDL receptor
    • LDLCQ2
    • Ldlr
    • LDLR_HUMAN
    • Low Density Lipoprotein Receptor
    • Low density lipoprotein receptor class A domain containing protein 3
    • Low density lipoprotein receptor familial hypercholesterolemia
    • Low-density lipoprotein receptor
    see all

  • Function

    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

  • Involvement in disease

    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).

  • Sequence similarities

    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.

  • Post-translational
    modifications

    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.

  • Cellular localization

    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)
    Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)
    Immobilized Human PCSK9 (D374Y) (His Tag) at 5µg/mL (100 µL/well) can bind Human LDL R (Fc Tag) with a linear range of 8-320 ng/mL (QC tested).
  • SDS-PAGE - Recombinant human LDL Receptor protein (Active) (ab220570)
    SDS-PAGE - Recombinant human LDL Receptor protein (Active) (ab220570)

    ab220570 on SDS-PAGE under reducing (lane 1) and no-reducing (lane 2) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.  As a result of glycosylation, the reduced protein migrates at 155-175 kDa and the non-reduced protein migrates at 310-350 kDa.

  • Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)
    Functional Studies - Recombinant human LDL Receptor protein (Active) (ab220570)

    Example of ab220570 bioactivity.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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