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Anti-GOLPH3/MIDAS antibody (ab236296)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GOLPH3/MIDAS antibody (ab236296)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GOLPH3/MIDAS
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GOLPH3/MIDAS antibody
    See all GOLPH3/MIDAS primary antibodies
  • Description

    Rabbit polyclonal to GOLPH3/MIDAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human GOLPH3/MIDAS aa 1-150.
    Database link: Q9H4A6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa, HEK-293 and A549 whole cell lysates. IHC-P: Human prostate cancer and prosate tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as GOLPH3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)

    Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-GOLPH3/MIDAS antibody (ab236296)
    Western blot - Anti-GOLPH3/MIDAS antibody (ab236296)
    All lanes : Anti-GOLPH3/MIDAS antibody (ab236296) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)

    Paraffin-embedded human prostate tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab236296)
    Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab236296)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GOLPH3/MIDAS(green) using ab236296 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

  • Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (ab236296)
    Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (ab236296)

    GOLPH3/MIDAS was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab236296 at 1/200 dilution. 

    Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.

    Lane 2: ab236296 IP in HeLa whole cell lysate.

    Lane 3: HeLa whole cell lysate 10 µg (Input).

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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