Anti-GOLPH3/MIDAS antibody (ab264406)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide within Human GOLPH3/MIDAS aa 1-50. The exact sequence is proprietary.
Database link: Q9H4A6 -
Positive control
- WB: HeLa, U-2 OS and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GOLPH3/MIDAS antibody (ab264406)All lanes : Anti-GOLPH3/MIDAS antibody (ab264406) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (ab264406)GOLPH3/MIDAS was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264406 at 100 µl per reaction. Western blot was performed from the immunoprecipitate using ab264406 at 1/1000 dilution.
Lane 1: ab264406 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
