Anti-GOLPH3/MIDAS antibody (ab91492)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatWB Rat
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Immunogen
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
Database link: AAH12123 -
General notes
This product was previously labelled as GOLPH3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml
All lanes : rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492) Image courtesy of Armen Petrosyan by Abreview.ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.
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Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492) Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492) Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.
