Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

All lanes : Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (purified)

Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse cerebral cortex tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/Diluting buffer 5% NFDM/TBST.

Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.

Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).

Lane 1: Rat brain whole cell lysate 10μg.

Lane 2: ab40801 IP in rat brain whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40801 in rat brain whole cell lysate.

For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

 

 

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/1000 dilution (purified) + Human fetal brain lysate at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa



Blocking/Diluting buffer 5% NFDM/TBST

Immunohistochemical analysis of human breast adenocarcinoma sections labelling DARPP32 with unpurified ab40801 at a dilution of 1/50. 

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (unpurified) + Rat brain lysate at 10 µg

Predicted band size: 32 kDa
Observed band size: 32 kDa

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.