Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DARPP32 antibody [EP721Y] (ab40802)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP721Y] to DARPP32
  • Suitable for: ICC/IF, WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DARPP32 antibody [EP721Y]
    See all DARPP32 primary antibodies

  • Description

    Rabbit monoclonal [EP721Y] to DARPP32

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within DARPP32 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human brain lysate and human gastric adenocarcinoma. SH-SY5Y (human neuroblastoma) and human fetal forebrain tissue lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution + Human brain tissue lysates. at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 32 kDa
    Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)

    ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

    Negative control 1: PBS in place of primary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)

    ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1:250)
  • Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)
    Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)

    ab40802 immunoprecipitating DARPP32. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: Human fetal forebrain whole cell lysate (10ug)
    Lane 2: Human fetal forebrain whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate

  • Anti-DARPP32 antibody [EP721Y] (ab40802)
    Anti-DARPP32 antibody [EP721Y] (ab40802)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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