Anti-Bax antibody [2D2] (ab270742)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2D2] to Bax
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-Bax antibody [2D2]
See all Bax primary antibodies -
Description
Mouse monoclonal [2D2] to Bax -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and MCF7 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2D2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-Bax antibody [2D2] (ab270742) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution
Predicted band size: 21 kDa
Exposure time: 37 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Bax was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (2μg in 0.35mg lysates) with ab270742 at 15.9µg/ml. Western blot was performed from the immunoprecipitate using ab270742 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab270742 IP in HeLa whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab270742 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 secs. -