Mouse Flt-3 ELISA Kit (ab256395)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.915 ng/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Flt-3 ELISA Kit
See all IFIT3/P60 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3% Inter-assay Sample n Mean SD CV% Extract 7 2.9% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.915 ng/ml -
Range
1.56 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 109 97% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Flt-3 ELISA Kit (ab256395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Flt-3 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Flt-3 with 0.915 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Flt-3, also known as CD135, is a receptor type tyrosine kinase involved in the differentiation of hematopoietic cells. Binding of its ligand, FLT3L induces phosphorylation cascades through multiple kinases, including STAT5A and MAPK1. Flt-3 is used to differentiate multipotent progenitor cells and hematopoietic stem cells. Mutations in this gene are linked to acute myelogenous leukemia and its levels are negatively associated with survival. Given Flt-3’s importance in this disease, it is currently being investigated as a therapeutic target.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Flt-3 Capture Antibody 1 x 600µl 10X Mouse Flt-3 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse Flt-3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the IFIT family.
Contains 8 TPR repeats. - Information by UniProt
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Alternative names
- CIG 49
- CIG49
- GARG 49
see all -
Database links
- Entrez Gene: 15959 Mouse
- SwissProt: Q64345 Mouse
- Unigene: 426079 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Flt-3 standard curve in 1X Cell Extraction Buffer PTR.The Flt-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Flt-3 in mouse M1 cell extract based on a 200 µg/mL extract load.The concentrations of Flt-3 were measured in duplicate and interpolated from the Flt-3 standard curve and corrected for sample dilution. The interpolated, dilution factor corrected, values are plotted (mean +/- SD, n=2). The mean Flt-3 concentration was determined to be 40.5 ng/mL.
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Sandwich ELISA - Mouse Flt-3 ELISA Kit (ab256395)To learn more about the advantages of recombinant antibodies see here.
