Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18283] to Bax - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bax antibody [EPR18283] - BSA and Azide free
    See all Bax primary antibodies

  • Description

    Rabbit monoclonal [EPR18283] to Bax - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa and HT-29 whole cell lysates.

  • General notes

    Ab232479 is the carrier-free version of ab182733. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232479 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)
    Immunoprecipitation - Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)

    Bax was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182733 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182733 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10µg (Input).

    Lane 2: ab182733 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab182733 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182733).

  • Western blot - Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)
    Western blot - Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: BAX knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 µg)
    Lane 4: HT29 (human colorectal adenocarcinoma cell line) whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab182733 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab182733 was shown to recognize BAX when BAX knockout samples were used, along with additional cross-reactive bands. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Ab182733 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182733).

  • Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)
    Anti-Bax antibody [EPR18283] - BSA and Azide free (ab232479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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