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OliGlo™ Biotin FISH Kit (ab253403)

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Overview

  • Product name

    OliGlo™ Biotin FISH Kit
  • Product overview

    Fluorescence in situ hybridization (FISH) kit which directly labels the phosphate groups (terminal and backbone) on DNA using a biotin labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.

    This kit when used with the supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. This standard size kit is suitable for labeling up to 120 μg of nucleic acid. The trial size kit is suitable for labeling up to 35 μg of nucleic acid.

    This kit is sold by Marker Gene under product code M1624/M1625.

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 120 µg 35 µg
    3M Sodium Acetate 1 x 500µl 1 x 100µl
    Activating Resin 1 vial 1 vial
    Denaturation Buffer D 1 x 200µl 1 x 50µl
    DMF 1 x 200µl 1 x 50µl
    Hybridization Buffer H 1 x 750µl 1 x 150µl
    OliGlo™ Biotin Labeling Dye Precursor 1 vial 1 vial
    Renaturation Buffer R 1 x 200µl 1 x 50µl
    TE Buffer 1 x 500µl 1 x 100µl

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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