Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha Gal antibody [m86] - Chimeric (ab281899)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [m86] to Alpha Gal - Chimeric
  • Suitable for: WB, Flow Cyt, ICC
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Alpha Gal antibody [m86] - Chimeric

  • Description

    Human monoclonal [m86] to Alpha Gal - Chimeric

  • Host species

    Human

  • Specificity

    ab281899 binds to the alpha-gal epitope on glycoproteins and glycolipids.

  • Tested applications

    Suitable for: WB, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Tissue, cells or virus corresponding to Alpha Gal. Rabbit red cells injected into alpha 1,3 galactosyltransferase knock-out mice.

  • Positive control

    • WB: Rat spleen tissue lysate; Pig spleen tissue lysate. Flow cyt: RAW 264.7 cells. ICC: RAW 264.7 cells.

  • General notes

    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

    This antibody does not have a J-chain and therefore presents as a hexamer, rather than a pentamer.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Proclin 300
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Recombinant lectin column.

  • Clonality

    Monoclonal

  • Clone number

    m86

  • Isotype

    IgM

  • Light chain type

    kappa

Images

  • Immunocytochemistry - (ab281899)
    Immunocytochemistry - (ab281899)

    RAW264.7 cells stained for alpha-gal epitope (green) using the original Mouse IgM version of this antibody at 10 μg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml in ICC. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor® 488 secondary antibody.

  • Flow Cytometry - (ab281899)
    Flow Cytometry - (ab281899)

    Flow-cytometry analysis of RAW264.7 cells labeling Alpha gal with anti-unknown IgG antibody (isotype control, black line) or the the original Mouse IgM version of this antibody (blue line) at 1/100 dilution for 1 hour at room temperature. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at 1/1000 dilution and cells analysed using a flow-cytometer.

  • Western blot - (ab281899)
    Western blot - (ab281899)
    All lanes : The original Mouse IgM version of this antibody at 0.3 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Pig spleen tissue lysate

    Secondary
    All lanes : Anti-mouse secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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