Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, Flow Cyt, SDS-PAGE
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Product name
Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (Active)
See all SARS-CoV-2 Spike Glycoprotein RBD proteins and peptides -
Biological activity
Binding ability measured in a functional ELISA. Immobilized Human ACE2 protein at 2 ug/ml (100 ul/well) can bind to ab273065.
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Purity
> 90 % SDS-PAGE. -
Expression system
CHO cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVL YNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDI STEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKCVNFGSGGGSGGHHHHHH -
Actual molecular weight
39 kDa including tags -
Amino acids
319 to 541 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)
SDS-PAGE analysis of ab273065.
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Flow Cytometry - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)Flow cytometry assay showing ab273065 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were labelledwith ab273065, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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Functional Studies - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)Binding ability measured in a funstional ELISA. Immobilized Human ACE2 protein at 2 ug/ml (100 ul/well) can bind to ab273065.
