Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric
  • Suitable for: ELISA, WB, Functional Studies

Overview

  • Product name

    Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric
    See all SARS-CoV-2 spike glycoprotein primary antibodies

  • Description

    Human monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric

  • Host species

    Human

  • Specificity

    We have in-house data that suggests this product also reacts with SARS-CoV-1 spike protein in Western blot.

  • Tested applications

    Suitable for: ELISA, WB, Functional Studiesmore details

  • Species reactivity

    Reacts with: SARS-CoV, SARS-CoV-2

  • Immunogen

    Recombinant fragment corresponding to Human SARS-CoV-2 spike glycoprotein aa 16-685.
    Database link: P0DTC2

  • Positive control

    • WB: SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate.

  • General notes

    Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.

    As the Fc region is human, an anti-Human secondary antibody should be used for detection.

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Constituents: 0.03% Proclin 300, 50% Glycerol, PBS
  • Concentration information loading...

  • Purity

    >90% by SDS-PAGE

  • Purification notes

    Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.

  • Clonality

    Monoclonal

  • Clone number

    H6

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) at 1 µg/ml

    Lane 1 : Mock transfected Expi cell lysate
    Lane 2 : PDL-1 Flag transfected Expi cell lysate
    Lane 3 : SARS-Cov1 spike protein transfected Expi cell lysate
    Lane 4 : SARS-Cov1 3xFlag spike protein transfected Expi cell lysate
    Lane 5 : SARS-Cov2 spike protein transfected Expi cell lysate
    Lane 6 : SARS-Cov2 3xFlag spike protein transfected Expi cell lysate
    Lane 7 : MERS Spike protein transfected Expi cell lysate
    Lane 8 : MERS 3xFlag Spike protein transfected Expi cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 8: Merged signal (red and green). Green - ab272854 observed at 200 kDa. Red - loading control.

    ab272854 was shown to react with SARS-CoV-2 spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab272854 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

    Binding Activity of ab272854 with SARS-CoV-2 spike glycoprotein.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 spike glycoprotein at 2 μg/ml can bind ab272854. The EC50 of ab272854 is 36.79-48.87 ng/ml.            

  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

    Binding Activity of ab272854 with SARS-CoV-2-S1-RBD
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind ab272854, the EC50 of SARS-CoV-2-S Antibody is 19.60-39.42 ng/ml.

  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

    Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 23.32 nM.

  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

    Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 2.38 µg/mL.

  • Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)
    Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Chimeric (ab272854)

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