SARS-CoV-2 (COVID-19) IgG ELISA Kit (ab277285)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 50ml Cut-off control 1 x 3ml HRP-human IgG antibody 1 x 20ml IgG Sample Dilution Buffer 1 x 100ml Negative Control 1 x 2ml Positive Control 1 x 2ml SARS-CoV-2 coated microplate 1 unit Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
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Relevance
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. -
Alternative names
- 2019-nCoV nucleocapsid protein
- N
- NC
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Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.