Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (Mutant) (ab274432)
Key features and details
- Expression system: Expi293 F cells
- Purity: >= 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (Mutant)
See all SARS-CoV-2 spike glycoprotein proteins and peptides -
Purity
>= 90 % SDS-PAGE. -
Endotoxin level
Expression system
Expi293 F cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Human coronavirus -
Sequence
SQCVNLTTRT QLPPAYTNSF TRGVYYPDKV FRSSVLHSTQ DLFLPFFSNV TWFHAIHVSG TNGTKRFDNP VLPFNDGVYF ASTEKSNIIR GWIFGTTLDS KTQSLLIVNN ATNVVIKVCE FQFCNDPFLG VYYHKNNKSW MESEFRVYSS ANNCTFEYVS QPFLMDLEGK QGNFKNLREF VFKNIDGYFK IYSKHTPINL VRDLPQGFSA LEPLVDLPIG INITRFQTLL ALHRSYLTPG DSSSGWTAGA AAYYVGYLQP RTFLLKYNEN GTITDAVDCA LDPLSETKCT LKSFTVEKGI YQTSNFRVQP TESIVRFPNI TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADYN YKLPDDFTGC VIAWNSNNLD SKVGGNYNYL YRLFRKSNLK PFERDISTEI YQAGSTPCNG VEGFNCYFPL QSYGFQPTNG VGYQPYRVVV LSFELLHAPA TVCGPKKSTN LVKNKCVNFN FNGLTGTGVL TESNKKFLPF QQFGRDIADT TDAVRDPQTL EILDITPCSF GGVSVITPGT NTSNQVAVLY QDVNCTEVPV AIHADQLTPT WRVYSTGSNV FQTRAGCLIG AEHVNNSYEC DIPIGAGICA SYQTQTNSPG SASSVASQSI IAYTMSLGAE NSVAYSNNSI AIPTNFTISV TTEILPVSMT KTSVDCTMYI CGDSTECSNL LLQYGSFCTQ LNRALTGIAV EQDKNTQEVF AQVKQIYKTP PIKDFGGFNF SQILPDPSKP SKRSFIEDLL FNKVTLADAG FIKQYGDCLG DIAARDLICA QKFNGLTVLP PLLTDEMIAQ YTSALLAGTI TSGWTFGAGA ALQIPFAMQM AYRFNGIGVT QNVLYENQKL IANQFNSAIG KIQDSLSSTA SALGKLQDVV NQNAQALNTL VKQLSSNFGA ISSVLNDILS RLDPPEAEVQ IDRLITGRLQ SLQTYVTQQL IRAAEIRASA NLAATKMSEC VLGQSKRVDF CGKGYHLMSF PQSAPHGVVF LHVTYVPAQE KNFTTAPAIC HDGKAHFPRE GVFVSNGTHW FVTQRNFYEP QIITTDNTFV SGNCDVVIGI VNNTVYDPLQ PELDSFKEEL DKYFKNHTSP DVDLGDISGI NASVVNIQKE IDRLNEVAKN LNESLIDLQE LGKYEQ -
Predicted molecular weight
140 kDa including tags -
Amino acids
13 to 1208 -
Modifications
mutated R682G + R683S + R685S + K986P + V987P -
Tags
His tag C-Terminus -
Additional sequence information
With stabilising mutations. C-terminal trimerisation domain (Leucine zipper), TEV cleavage site, 10x His-tag.
Description
Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (mutated R682G + R683S + R685S + K986P + V987P) (Mutant)Specifications
Our Abpromise guarantee covers the use of ab274432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Information available upon request.
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ReconstitutionReconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
Images
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SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (Mutant) (ab274432)
SDS-PAGE analysis of ab274432.
Band expected at 140kDa +/- 10%.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab274432 has not yet been referenced specifically in any publications.
Preparation and Storage
Images
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SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 spike glycoprotein (Mutant) (ab274432)
SDS-PAGE analysis of ab274432.
Band expected at 140kDa +/- 10%.