Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)
![Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-388068-Ant-sars-spike-glycoprotein-antibody-3a2-immunofluorescence-sars-infected-vero-e6-cells-coronavirus.jpg "Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)")
Key features and details
- Mouse monoclonal [3A2] to SARS spike glycoprotein - Coronavirus
- Suitable for: WB, ICC
- Isotype: IgG2b
Overview
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Product name
Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus
See all SARS spike glycoprotein primary antibodies -
Description
Mouse monoclonal [3A2] to SARS spike glycoprotein - Coronavirus -
Host species
Mouse -
Specificity
We have received mixed feedback regarding the specificity of this antibody against SARS-Cov2, please see the customer reviews section.
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Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: SARS-CoV
Does not react with: MERS-CoV -
Immunogen
Tissue, cells or virus corresponding to Human coronavirus SARS spike glycoprotein. (Formaldehyde inactivated SARS Coronavirus).
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Positive control
- ICC: SARS virus infected Vero E6 cells. WB: SARS virus infected cells.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data Reviews: Customers have been successful and shared their AbReview
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3A2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [3A2] (ab272420)
Immunofluorescence analysis of SARS spike glycoprotein in the SARS virus infected cells with ab272420 antibody at 1/1000 dilution. (a) Uninfected Vero E6 cells. (b) SARS virus infected Vero E6 cells.
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![Western blot - Anti-SARS spike glycoprotein antibody [3A2] (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-388069-Ant-sars-spike-glycoprotein-antibody-3a2-western-blot-sars-infected-cells-coronavirus.jpg)
Western blot - Anti-SARS spike glycoprotein antibody [3A2] (ab272420)Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420) at 1/10000 dilution + Crude extract of the SARS virus infected cells
Predicted band size: 139 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-392810-anti-sars-spike-glycoprotein-antibody-3a2-coronavirus-immunocytochemistry-vera-infected-sars-cov2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)Immunofluorescence analysis of SARS spike glycoprotein in Vero cells infected with the SARS-CoV2 virus.
Left image: ab272420 (1 µg/mL)
Right image: Anti-SARS-CoV2 N protein monoclonal antibody
An Alexa 488-conjugated goat anti-mouse IgG (H+L) (1/2000) was used as the secondary antibody.
ab272420 does not cross-react with SARS-CoV2.
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![Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)](https://www.abcam.com/ps/products/272/ab272420/Images/ab272420-392812-anti-sars-spike-glycoprotein-antibody-3a2-coronavirus-immunocytochemistry-vera-infected-mers-cov.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)Immunofluorescence analysis of SARS spike glycoprotein in Vero cells infected with the MERS-CoV virus.
Left image: ab272420 (1 µg/mL)
Right image: Anti-MERS-CoV N protein monoclonal antibody
An Alexa 488-conjugated goat anti-mouse IgG (H+L) (1/2000) was used as the secondary antibody.
ab272420 does not cross-react with MERS-CoV.