Anti-SNRP70/U1-70K antibody (ab83306)
Key features and details
- Rabbit polyclonal to SNRP70/U1-70K
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-SNRP70/U1-70K antibody -
Description
Rabbit polyclonal to SNRP70/U1-70K -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
-
Immunogen
Synthetic peptide corresponding to Human SNRP70/U1-70K aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab95039) -
Positive control
- This antibody gave a positive signal in Mouse Skeletal muscle and Mouse Heart tissue lysates as wellas the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; CaCo2; MCF7; SHSY5Y; NIH3T3; MEF1. IHC-P: Normal Human Colon Tissue
-
General notes
This product was previously labelled as SNRP70
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa). Target
-
Relevance
SNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6625 Human
- Entrez Gene: 20637 Mouse
- Entrez Gene: 361574 Rat
- Omim: 180740 Human
- SwissProt: Q1RMR2 Cow
- SwissProt: P08621 Human
- SwissProt: Q62376 Mouse
- Unigene: 467097 Human
-
Alternative names
- RNPU1Z antibody
- Rnulp70 antibody
- RPU1 antibody
see all
Images
-
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)All lanes : Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The 63 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human U1 small nuclear ribonucleoprotein 70 kDa. -
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)All lanes : Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/ml
Lane 1 : Testis (Rat) Tissue Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody (ab83306)IHC image of SNRP70/U1-70K antibody staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab83306, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
Immunocytochemistry/ Immunofluorescence - Anti-SNRP70/U1-70K antibody (ab83306)ICC/IF image of ab83306 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83306, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and Hek293 cells at 5µg/ml.
Protocols
Datasheets and documents
References (8)
ab83306 has been referenced in 8 publications.
- Hemmer MC et al. E47 modulates hepatic glucocorticoid action. Nat Commun 10:306 (2019). PubMed: 30659202
- Leisegang MS et al. The histone demethylase PHF8 facilitates alternative splicing of the histocompatibility antigen HLA-G. FEBS Lett 593:487-498 (2019). PubMed: 30758047
- Antonova A et al. Heat-Shock Protein 90 Controls the Expression of Cell-Cycle Genes by Stabilizing Metazoan-Specific Host-Cell Factor HCFC1. Cell Rep 29:1645-1659.e9 (2019). PubMed: 31693902
- Wang Z et al. lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer. Cancer Cell 33:706-720.e9 (2018). PubMed: 29622465
- Rogalska ME et al. Therapeutic activity of modified U1 core spliceosomal particles. Nat Commun 7:11168 (2016). PubMed: 27041075
- Schmidt K et al. The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Cell Rep 15:2025-37 (2016). PubMed: 27210747
- Franco HL et al. TNFa signaling exposes latent estrogen receptor binding sites to alter the breast cancer cell transcriptome. Mol Cell 58:21-34 (2015). Human . PubMed: 25752574
- Mozzetti S et al. Gli family transcription factors are drivers of patupilone resistance in ovarian cancer. Biochem Pharmacol 84:1409-18 (2012). PubMed: 22964220
Images
-
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)All lanes : Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The 63 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human U1 small nuclear ribonucleoprotein 70 kDa. -
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)All lanes : Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/ml
Lane 1 : Testis (Rat) Tissue Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody (ab83306)
IHC image of SNRP70/U1-70K antibody staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab83306, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
Immunocytochemistry/ Immunofluorescence - Anti-SNRP70/U1-70K antibody (ab83306)ICC/IF image of ab83306 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83306, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and Hek293 cells at 5µg/ml.