Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157)
![Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157)](https://www.abcam.com/ps/products/282/ab282157/Images/ab282157-2-anti-human-SUMO23-antibody-AT10F1-wb-human-cells.jpg "Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AT10F1] to Sumo 2 + Sumo 3
- Suitable for: ICC, Flow Cyt (Intra), WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Sumo 2 + Sumo 3 antibody [AT10F1]
See all Sumo 2 + Sumo 3 primary antibodies -
Description
Mouse monoclonal [AT10F1] to Sumo 2 + Sumo 3 -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human Sumo 2 + Sumo 3 aa 1-100.
Database link: P61956 -
Positive control
- ICC: HeLa cells. WB: HeLa, Jurkat, K562 and HL-60 cell lysate, Recombinant human SUMO2 protein, Recombinant human SUMO3 protein. FC: Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.75% Sodium chloride, 0.02% Potassium chloride, 0.13% Dibasic monohydrogen sodium phosphate, 0.02% Monobasic dihydrogen potassium phosphate, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT10F1 -
Isotype
IgG2b -
Light chain type
kappa
Images
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Western blot - Anti-Sumo 2 + Sumo 3 antibody (ab282157)All lanes : Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Mouse secondary antibody HRP-conjugate
Predicted band size: 12 kDaSDS-PAGE gel was transferred to PVDF membrane.
ECL detection system.
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Western blot - Anti-Sumo 2 + Sumo 3 antibody (ab282157)All lanes : Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157) at 1/1000 dilution
Lane 1 : Recombinant human SUMO1 protein
Lane 2 : Recombinant human SUMO2 protein
Lane 3 : Recombinant human SUMO3 protein
Lysates/proteins at 0.001 µg per lane.
Secondary
All lanes : Goat anti-Mouse secondary antibody HRP-conjugate
Predicted band size: 12 kDaSDS-PAGE gel was transferred to PVDF membrane.
ECL detection system.
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Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody (ab282157)Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SUMO2/3 with ab282157 at 1/100 dilution followed by Alexa Fluor® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).
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Flow Cytometry - Anti-Sumo 2 + Sumo 3 antibody (ab282157)Flow cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labelling SUMO2/3 with ab282157 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa Fluor® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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![Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157)](https://www.abcam.com/ps/products/282/ab282157/Images/ab282157-464273-benefitsofrecombinantantibodies.jpg)
Anti-Sumo 2 + Sumo 3 antibody [AT10F1] (ab282157)