Human PBK (SPK) knockout HEK-293T cell lysate (ab257575)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261015 - Human PBK knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human PBK (SPK) knockout HEK293T cell lysate (ab257575)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PBK knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab236871 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab236871 Anti-PBK/SPK antibody [EPR21982] was shown to specifically react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266827 (knockout cell lysate ab257575) was used. Wild-type and PBK/SPK knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab236871 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PBK (SPK) knockout HEK293T cell lysate (ab257575)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PBK knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab75987 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab75987 Anti-PBK/SPK antibody [EP2520Y] was shown to specifically react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266827 (knockout cell lysate ab257575) was used. Wild-type and PBK/SPK knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75987 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 100000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PBK knockout HEK293T cell lysate (ab257575)Homozygous: 1 bp deletion in exon 2