Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)
![Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)](https://www.abcam.com/ps/products/251/ab251208/Images/ab251208-2-anti-histone-h2a-crotonyl-k119-antibody-epr18769-bsa-and-azide-free-dot-blot.jpg "Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18769] to Histone H2A (crotonyl K119) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EPR18769] to Histone H2A (crotonyl K119) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251208 is the carrier-free version of ab195467. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251208 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18769 -
Isotype
IgG
Images
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Dot Blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)This data was developed using ab195467, the same antibody clone in a different buffer formulation.Dot blot analysis of H2A K119 crotonyl peptide (Lane 1), H2A K119 ubiquitinate peptide (Lane 2), H2A.X K119 ubiquitinate peptide (Lane 3), H2A K118 formyl peptide (Lane 4), H2A.X unmodified peptide (Lane 5) and H2A unmodified peptide (Lane 6), labeled using ab195467 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H+L (HRP) (ab97051) secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% BSA/TBST.Exposure time: 3 minutes.
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![Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)](https://www.abcam.com/ps/products/251/ab251208/Images/ab251208-399037-anti-histone-h2a-crotonyl-k119-antibody-epr18769-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsThis data was developed using ab195467, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% BSA/TBST.
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![Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)](https://www.abcam.com/ps/products/251/ab251208/Images/ab251208-3-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (crotonyl K119) antibody [EPR18769] - BSA and Azide free (ab251208)