Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human KIR2DL1 (aa1-245), His and LIF-tagged human KIR2DL2 (aa26-221), His-tagged human KIR2DL3 (aa1-245), His-tagged human KIR2DS1 (aa22-221) and His-tagged human KIR2DS2 recombinant proteins (aa22-221). NK-92 treated with 5-aza-2-deoxycytidine whole cell lysate. IHC-P: Human colon cancer, gastric cancer and endometrium tissue. ICC/IF: NK-92 cells treated with 5-aza-2-deoxycytidine. Flow Cyt: Human PBMC. IP: NK-92 treated with 5-aza-2-deoxycytidine, whole cell lysate

  • General notes

    Ab255327 is the carrier-free version of ab252938. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Immunocytochemistry/ Immunofluorescence - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling KIR2DL1 with ab252938 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine(2µM) for 48hrs. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Flow Cytometry - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Flow Cytometry - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling KIR2DL1 with ab252938 at 1/50 (right) compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252938 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human colon cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Immunoprecipitation - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Immunoprecipitation - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    KIR2DL1 was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with 2μM 5-aza-2-deoxycytidine for 48hrs, whole cell lysate with ab252938 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252938 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: NK-92 whole cell lysate (treated as above) 10 µg (Input).
    Lane 2: ab252938 IP in NK-92 whole cell lysate (treated as above).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252938 in NK-92 whole cell lysate (treated as above).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human gastric cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (ab255327)
    Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (ab255327)

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