Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
![Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-445657-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-western-blot.jpg "Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free
See all Bad primary antibodies -
Description
Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IP, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab171725) -
General notes
Ab248343 is the carrier-free version of ab129192. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 4.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1891(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)
Lane 1 : Untreated C6 cells
Lane 2 : C6 cells treated with Calcyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?This data was developed using ab129192, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-5-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)This data was developed using ab129192, the same antibody clone in a different buffer formulation.ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST. -
![Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-4-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)This data was developed using ab129192, the same antibody clone in a different buffer formulation.Dot blot analysis of Bad (phospho S112) using ab129192 primary antibody at a dilution of 1/1000. ab97051 was used as a secondary antibody at a dilution of 1/100,000. Lane 1: Bad (pS112) phospho peptide
Lane 2: Bad non-phospho peptide Blocking and diluting buffer: 5% NFDM/TBST -
![Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-405669-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)
Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Calcyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?This data was developed using ab129192, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-402409-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDaThis data was developed using ab129192, the same antibody clone in a different buffer formulation.
-
![OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-1-anti-bad-phospho-s112-antibody-epr1891-2-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)This data was developed using ab129192, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
-
![Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)](https://www.abcam.com/ps/products/248/ab248343/Images/ab248343-6-benefits-of-recombinant-antibodies.png)
Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)