Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free
    See all Bad primary antibodies

  • Description

    Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab171725)

  • General notes

    Ab248343 is the carrier-free version of ab129192. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)

    Lane 1 : Untreated C6 cells
    Lane 2 : C6 cells treated with Calcyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab129192, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    This data was developed using ab129192, the same antibody clone in a different buffer formulation.ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    This data was developed using ab129192, the same antibody clone in a different buffer formulation.Dot blot analysis of Bad (phospho S112) using ab129192 primary antibody at a dilution of 1/1000. ab97051 was used as a secondary antibody at a dilution of 1/100,000. Lane 1: Bad (pS112) phospho peptide
    Lane 2: Bad non-phospho peptide Blocking and diluting buffer: 5% NFDM/TBST
  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)

    Lane 1 : Untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with Calcyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    This data was developed using ab129192, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilution

    Lane 1 : HeLa cell lysates, untreated
    Lane 2 : HeLa cell lysates, treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa



    This data was developed using ab129192, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    This data was developed using ab129192, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)
    Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free (ab248343)

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