Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR066G] to CHD4
Suitable for: IP, WB
Reacts with: Mouse, Human

Product name

Anti-CHD4 antibody [BLR066G]
See all CHD4 primary antibodies
Description

Rabbit monoclonal [BLR066G] to CHD4
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human CHD4 aa 25-75. The exact sequence is proprietary.
Database link: Q14839
Positive control
- WB: MCF7, A549, HeLa, K562, Jurkat, TCMK-1 and RKO cell lysate. IP: HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR066G
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CHD4 antibody [BLR066G] (ab264417)

CHD4 was immunoprecipitated from 1 mg HEK-293T whole cell lysate using ab264417 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab264417 at 1/1000 dilution.

Lane 1: ab264417 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Western blot - Anti-CHD4 antibody [BLR066G] (ab264417)

Anti-CHD4 antibody [BLR066G] (ab264417) at 1/1000 dilution + TCMK-1 whole cell lysate at 50 µg

Secondary
HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Exposure time: 75 seconds

Cells prepared using NETN lysis buffer.
Western blot - Anti-CHD4 antibody [BLR066G] (ab264417)

All lanes : Anti-CHD4 antibody [BLR066G] (ab264417) at 1/1000 dilution

Lane 1 : MCF7 whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : RKO whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Rabbit anti-GAPDH

Developed using the ECL technique.

Exposure time: 10 seconds
Anti-CHD4 antibody [BLR066G] (ab264417)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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