Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHD4 antibody [EPR22953-38] - ChIP Grade – BSA and Azide free (ab263025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22953-38] to CHD4 - ChIP Grade – BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF, ChIP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CHD4 antibody [EPR22953-38] - ChIP Grade – BSA and Azide free
    See all CHD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22953-38] to CHD4 - ChIP Grade – BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF, ChIPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT, K562, HeLa, HEK-293 and RAW264.7 lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa cells. IP: NCCIT cells. ChIP: Chromatin prepared from K562 cells.

  • General notes

    ab263025 is the carrier-free version of ab240640.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    ChIP - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    Chromatin was prepared from K-562 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab240640 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are from paper PMID: 23505388.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240640).

  • Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    Immunoprecipitation - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)

    CHD4 was immunoprecipitated from 0.35 mg NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug with ab240640 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240640 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NCCIT whole cell lysate 10ug.

    Lane 2: ab240640 IP in NCCIT whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240640 in NCCIT whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240640).

  • Flow Cytometry - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    Flow Cytometry - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CHD4 with ab240640 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240640).

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeing CHD4 with ab240640 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining and weak cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240640).

  • Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)
    Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR22953-38] - BSA and Azide free (ab263025)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CHD4 with ab240640 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining and weak cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240640).

  • Anti-CHD4 antibody [EPR22953-38] - ChIP Grade – BSA and Azide free (ab263025)
    Anti-CHD4 antibody [EPR22953-38] - ChIP Grade – BSA and Azide free (ab263025)

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