Anti-CHD4 antibody [EPR12229] (ab181370)
![Anti-CHD4 antibody [EPR12229] (ab181370)](https://www.abcam.com/ps/products/181/ab181370/Images/ab181370-210731-anti-chd4-antibody-epr12229-western-blot.jpg "Anti-CHD4 antibody [EPR12229] (ab181370)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12229] to CHD4
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-CHD4 antibody [EPR12229]
See all CHD4 primary antibodies -
Description
Rabbit monoclonal [EPR12229] to CHD4 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 95% homology with CDH3. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human CHD4 aa 1800-1900. The exact sequence is proprietary.
Database link: Q14839 -
Positive control
- NCCIT, 293T, HeLa and Jurkat whole cell lysate (ab7899); HeLa cells; Permeabilized 293T cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12229 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHD4 antibody [EPR12229] (ab181370)All lanes : Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilution
Lane 1 : NCCIT cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 218 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR12229] (ab181370)](https://www.abcam.com/ps/products/181/ab181370/Images/ab181370-210729-anti-chd4-antibody-epr12229-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHD4 antibody [EPR12229] (ab181370)Immunofluorescent analysis of HeLa cells labeling CHD4 with ab181370 at 1/50 dilution.
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![Flow Cytometry - Anti-CHD4 antibody [EPR12229] (ab181370)](https://www.abcam.com/ps/products/181/ab181370/Images/ab181370-210727-anti-chd4-antibody-epr12229-flow-cytometry.jpg)
Flow Cytometry - Anti-CHD4 antibody [EPR12229] (ab181370)Flow cytometric analysis of permeabilized 293T cells labeling CHD4 with ab181370 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-CHD4 antibody [EPR12229] (ab181370)](https://www.abcam.com/ps/products/181/ab181370/Images/ab181370-4-benefits-of-recombinant-antibodies.png)
Anti-CHD4 antibody [EPR12229] (ab181370)
