Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PON2 antibody [EPR18547-2] (ab192038)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18547-2] to PON2
Suitable for: WB, Flow Cyt, IP
Reacts with: Mouse, Rat

Product name

Anti-PON2 antibody [EPR18547-2]
See all PON2 primary antibodies
Description

Rabbit monoclonal [EPR18547-2] to PON2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse PON2 (aa1-354) recombinant protein; mouse heart and lung tissue lysates; rat liver, lung and heart tissue lysates; L-929 whole cell lysate. IP: L-929 whole cell lysate. Flow cytometry: L-929 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18547-2
Isotype

IgG
Research areas

Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038)

All lanes : Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1 : GST-His-tagged mouse PON1 (aa2-355) recombinant protein, 10 ng
Lane 2 : His-tagged mouse PON2 (aa1-354) recombinant protein , 10 ng
Lane 3 : His-tagged mouse PON3 (aa2-354) recombinant protein, 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa

Exposure time: 6 seconds

Blocking and dilution buffer: 5% NFDM/TBST

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Flow Cytometry - Anti-PON2 antibody [EPR18547-2] (ab192038)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized L-929 (mouse connective tissue fibroblast) cell line labeling PON2 with ab192038 at 1/600 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-PON2 antibody [EPR18547-2] (ab192038)

PON2 was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab192038 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: L-929 whole cell lysate 10 μg (Input).

Lane 2: ab192038 IP in L-929 whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192038 in L-929 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038)

All lanes : Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST
Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038)

All lanes : Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1 : Rat liver tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Blocking and dilution buffer: 5% NFDM/TBST

Exposure times.

Lane 1: 24 seconds

Lanes 2 & 3: 3 minutes

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Anti-PON2 antibody [EPR18547-2] (ab192038)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com