Anti-PON2 antibody (ab40969)
Key features and details
- Rabbit polyclonal to PON2
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PON2 antibody
See all PON2 primary antibodies -
Description
Rabbit polyclonal to PON2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON2.
Read Abcam's proprietary immunogen policy (Peptide available as ab41542.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseRatChickenCowDogApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. -
Tissue specificity
Widely expressed with highest expression in liver, lung, placenta, testis and heart. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 395830 Chicken
- Entrez Gene: 281417 Cow
- Entrez Gene: 403855 Dog
- Entrez Gene: 5445 Human
- Entrez Gene: 330260 Mouse
- Entrez Gene: 296851 Rat
- Omim: 602447 Human
- SwissProt: Q90952 Chicken
see all -
Alternative names
- A esterase 2 antibody
- A-esterase 2 antibody
- Aromatic esterase 2 antibody
see all
Images
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Western blot - Anti-PON2 antibody (ab40969)All lanes : Anti-PON2 antibody (ab40969) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PON2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab40969 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.
ab40969 Anti-PON2 antibody was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab40969 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody (ab40969)ICC/IF image of ab40969 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-PON2 antibody (ab40969)All lanes : Anti-PON2 antibody (ab40969) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible glycosylated form), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-PON2 antibody (ab40969)
Protocols
Datasheets and documents
References (3)
ab40969 has been referenced in 3 publications.
- Shi S et al. Regulation of the epithelial Na+ channel by paraoxonase-2. J Biol Chem 292:15927-15938 (2017). PubMed: 28768768
- Birkus G et al. Intracellular Activation of Tenofovir Alafenamide and the Effect of Viral and Host Protease Inhibitors. Antimicrob Agents Chemother 60:316-22 (2015). PubMed: 26503655
- Giordano G et al. Gender differences in brain susceptibility to oxidative stress are mediated by levels of paraoxonase-2 expression. Free Radic Biol Med 58:98-108 (2013). WB ; Mouse, Human . PubMed: 23376469
Images
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Western blot - Anti-PON2 antibody (ab40969)All lanes : Anti-PON2 antibody (ab40969) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PON2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab40969 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.
ab40969 Anti-PON2 antibody was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab40969 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody (ab40969)ICC/IF image of ab40969 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-PON2 antibody (ab40969)All lanes : Anti-PON2 antibody (ab40969) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible glycosylated form), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-PON2 antibody (ab40969)
