Human Osteocalcin ELISA Kit (ab270202)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.99 pg/ml
- Range: 52.08 pg/ml - 3333 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Osteocalcin ELISA Kit
See all Osteocalcin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.1% Inter-assay Sample n Mean SD CV% Serum 3 7% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13.99 pg/ml -
Range
52.08 pg/ml - 3333 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 80% - 103% Cell culture media 102 91% - 115% Hep Plasma 93 81% - 99% EDTA Plasma 93 90% - 96% Cit plasma 90 84% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Osteocalcin ELISA Kit (ab270202) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteocalcin protein in cell culture media, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Osteocalcin with 13.99 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Osteocalcin is a small secreted protein 49 amino acids produced by osteoblasts. Osteocalcin acts as a hormone impacting bone mineral absorption, glucose metabolism and insulin secretion. During bone reabsorption osteoclasts break down the bone matrix releasing bound Osteocalcin into 5 different fragments; N-terminal, Mid, C-terminal, N-terminal-Mid, and Mid-C-terminal into the serum. Circulating levels of intact Osteocalcin and Osteocalcin fragments have been found to vary significantly between healthy individuals and diseased stats such as Paget’s disease, osteoporosis, fracture, and renal failure. The standard protein in this product is mature Osteocalcin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Osteocalcin Capture Antibody 1 x 600µl 10X Human Osteocalcin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Osteocalcin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. -
Sequence similarities
Belongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BGLAP
- BGP
- Bone gamma carboxyglutamate (gla) protein
see all -
Database links
- Entrez Gene: 632 Human
- Omim: 112260 Human
- SwissProt: P02818 Human
- Unigene: 654541 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Osteocalcin competitor curve comparisonStandard Curve comparison between human osteocalcin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human osteocalcin standard curve comparisonStandard Curve comparison between the original Human Ostecalcin SimpleStep ELISA kit (ab270202) and current Human Ostecalcin SimpleStep ELISA kit (ab195214).
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Example of human Osteocalcin standard curve in Sample Diluent NS.The Osteocalcin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Osteocalcin in human serum and plasma samples.The concentrations of Osteocalcin were measured in duplicates, interpolated from the Osteocalcin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 10%, plasma (heparin) 7.5%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 34.49 ng/mL in serum, 24.94 ng/mL in plasma (citrate), 41.51 ng/ml in plasma (heparin), and 32.69 ng/ml in plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 18.95 ng/mL with a range of 1.30 – 38.94 ng/mL.
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Sandwich ELISA - Human Osteocalcin ELISA Kit (ab270202)To learn more about the advantages of recombinant antibodies see here.
