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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Bone

Human Osteocalcin Antibody Pair - BSA and Azide free (ab253302)

Human Osteocalcin Antibody Pair - BSA and Azide free (ab253302)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Osteocalcin Antibody Pair - BSA and Azide free
    See all Osteocalcin kits
  • Assay type

    ELISA set
  • Range

    52.07 pg/ml - 3333 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Osteocalcin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270202), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Osteocalcin Capture Antibody (unconjugated) 1 x 100µg
    Human Osteocalcin Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
  • Function

    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities

    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02818 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • BGLAP
    • BGP
    • Bone gamma carboxyglutamate (gla) protein
    • Bone gamma carboxyglutamate gla protein osteocalcin
    • Bone gamma carboxyglutamate protein
    • Bone Gla protein
    • Gamma carboxyglutamic acid containing protein
    • Gamma-carboxyglutamic acid-containing protein
    • OC
    • OCN
    • OSTCN_HUMAN
    • Osteocalcin
    • OTTHUMP00000016586
    • PMF1
    see all
  • Database links

    • Entrez Gene: 632 Human
    • Omim: 112260 Human
    • SwissProt: P02818 Human
    • Unigene: 654541 Human

    Images

    • Sandwich ELISA - Human Osteocalcin Antibody Pair - BSA and Azide free (ab253302)
      Sandwich ELISA - Human Osteocalcin Antibody Pair - BSA and Azide free (ab253302)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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      Applications:

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