Human FRZB ELISA Kit (sFRP-3) (ab267653)
Key features and details
- Sensitivity: 190 pg/ml
- Range: 190 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FRZB ELISA Kit (sFRP-3) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
190 pg/ml -
Range
190 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100.1 91% - 108% Plasma 76.99 71% - 89% Cell culture media 91.66 78% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FRZB ELISA Kit (sFRP-3) is designed for the quantitative determination of FRZB in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human FRZB coated on a 96-well plate. Standards and samples are pipetted into the wells and FRZB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FRZB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRZB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human 2B4, ADAM-9, APRIL, BMP-2, BMP-9, DcR3, FABP-2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha/betaR2, IGF-2R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRPs, Serpin A4, Thrombomodulin, TLP2, Transferrin, WIF-1.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human FRZB coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human FRZB Detection Antibody 2 vials Human FRZB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
SFRP3/FRZB appears to be involved in limb skeletogenesis by regulating chondrocyte maturation and long bone development. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. Antagonist of Wnt8 signaling. -
Cellular localization
Secreted -
Alternative names
- FIZ
- FRE
- Frezzled
see all -
Database links
- Entrez Gene: 2487 Human
- Omim: 605083 Human
- SwissProt: Q92765 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.