Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TSPEAR antibody [EPR15505] (ab192023)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15505] to TSPEAR
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-TSPEAR antibody [EPR15505]
See all TSPEAR primary antibodies
Description

Rabbit monoclonal [EPR15505] to TSPEAR
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, C6, RAW264.7 and 293 cell lysate. Human testis lysate. Human kidney and stomach tissue. A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15505
Isotype

IgG

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

Predicted band size: 75 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?
Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/5000 dilution + human testis lysate at 1/10 dilution

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] (ab192023)

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain: ab7291 and ab150120 both at 1/500 dilution. The negative controls: Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.
Anti-TSPEAR antibody [EPR15505] (ab192023)

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