Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSPEAR antibody [EPR15505] (ab192023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15505] to TSPEAR
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSPEAR antibody [EPR15505]
    See all TSPEAR primary antibodies

  • Description

    Rabbit monoclonal [EPR15505] to TSPEAR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, C6, RAW264.7 and 293 cell lysate. Human testis lysate. Human kidney and stomach tissue. A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15505

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/5000 dilution + human testis lysate at 1/10 dilution

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)
    All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] (ab192023)
    Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain: ab7291 and ab150120 both at 1/500 dilution. The negative controls: Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.

  • Anti-TSPEAR antibody [EPR15505] (ab192023)
    Anti-TSPEAR antibody [EPR15505] (ab192023)

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