Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Jagged1 antibody [EPR4290] - BSA and Azide free
    See all Jagged1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.

  • General notes

    ab184785 is the carrier-free version of ab109536. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab184785 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : JAG1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 134 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 180 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

    Immunohistochemical staining of paraffin embedded human kidney using purified ab109536 at a dilution of 1/300. A pre-diluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

    Immunohistochemical staining of Jagged1 in paraffin-embedded bladder cancer tissue using unpurified ab109536 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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