Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Jagged1 antibody (ab85763)

Key features and details

  • Rabbit polyclonal to Jagged1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Jagged1 antibody
    See all Jagged1 primary antibodies

  • Description

    Rabbit polyclonal to Jagged1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish

  • Immunogen

    Synthetic peptide corresponding to Human Jagged1 aa 1200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab94375)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: MEL-1; HeLa; HEK293

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab85763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

    • Tissue specificity

      Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

    • Involvement in disease

      Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.

    • Sequence similarities

      Contains 1 DSL domain.
      Contains 15 EGF-like domains.

    • Developmental stage

      Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AGS antibody
      • AHD antibody
      • AWS antibody
      • CD 339 antibody
      • CD339 antibody
      • CD339 antigen antibody
      • Headturner antibody
      • hJ1 antibody
      • Htu antibody
      • Jag 1 antibody
      • Jag1 antibody
      • JAG1_HUMAN antibody
      • Jagged 1 antibody
      • Jagged1 (Alagille syndrome) antibody
      • Jagged1 antibody
      • JAGL1 antibody
      • MGC104644 antibody
      • OTTHUMP00000030278 antibody
      • Protein jagged-1 antibody
      • Ser 1 antibody
      • Ser1 antibody
      • Serrate 1 antibody
      • Slalom antibody
      see all

    Images

    • Western blot - Anti-Jagged1 antibody (ab85763)
      Western blot - Anti-Jagged1 antibody (ab85763)
      All lanes : Anti-Jagged1 antibody (ab85763) at 1 µg/ml

      Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 134 kDa
      Observed band size: 160 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 105 kDa, 40 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Jagged1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      ICC/IF image of ab85763 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      ICC/IF image of ab85763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (3)

    Publishing research using ab85763? Please let us know so that we can cite the reference in this datasheet.

    ab85763 has been referenced in 3 publications.

    • Li N  et al. Notch activity mediates oestrogen-induced stromal cell invasion in endometriosis. Reproduction 157:371-381 (2018). PubMed: 30753135
    • Labsch S  et al. Sulforaphane and TRAIL induce a synergistic elimination of advanced prostate cancer stem-like cells. Int J Oncol 44:1470-80 (2014). WB ; Human . PubMed: 24626333
    • Cuman C  et al. Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success. Hum Reprod 28:1161-71 (2013). Human . PubMed: 23477906

    Images

    • Western blot - Anti-Jagged1 antibody (ab85763)
      Western blot - Anti-Jagged1 antibody (ab85763)
      All lanes : Anti-Jagged1 antibody (ab85763) at 1 µg/ml

      Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 134 kDa
      Observed band size: 160 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 105 kDa, 40 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Jagged1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      ICC/IF image of ab85763 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab85763)
      ICC/IF image of ab85763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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