Anti-Ki67 antibody [B56] (ab279653)
![Anti-Ki67 antibody [B56] (ab279653)](https://www.abcam.com/ps/products/279/ab279653/Images/ab279653-1-anti-ki67-antibody-b56-immunohistochemistry-human-colon.jpg "Anti-Ki67 antibody [B56] (ab279653)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [B56] to Ki67
- Suitable for: ICC, Flow Cyt (Intra), IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Ki67 antibody [B56]
See all Ki67 primary antibodies -
Description
Mouse monoclonal [B56] to Ki67 -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt (Intra), IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon, ovarian carcinoma, Mouse colon, breast carcinoma, Rat colon tissues. ICC: HeLa cells. Flow Cyt: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
B56 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab279653)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon. The section was incubated with ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab279653)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Ki67 with ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human ovarian carcinoma. The section was incubated with ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunocytochemistry - Anti-Ki67 antibody (ab279653)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Ki67 with ab279653 at 1/50 (18.72 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunocytochemistry - Anti-Ki67 antibody [B56] (ab279653)](https://www.abcam.com/ps/products/279/ab279653/Images/ab279653-459243-anti-ki67-antibody-b56-immunocytochemistry-helaKO.jpg)
Immunocytochemistry - Anti-Ki67 antibody [B56] (ab279653)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized wild-type HeLa (Human cervix adenocarcinoma epithelial cell) cells and KI67 KO HeLa cells labelling Ki67 with ab279653 at 1/50 dilution (18.72 ug/ml), followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in Parental HeLa cell line and no staining in MKI67 HeLa KO cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution was used to counterstain tubulin (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow Cytometry - Anti-Ki67 antibody (ab279653)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Ki67 with ab279653 at 1/1000 dilution (0.1ug)/ Right compared with a Mouse monoclonal IgG / Left isotype control. A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry - Anti-Ki67 antibody (ab279653)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Parental HeLa (Human cervix adenocarcinoma epithelial cells) (Right)/ MKI67 KO HeLa cells (Left) labelling Ki67 with ab279653 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab279653)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Ki67 with ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse colon. The section was incubated with ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab279653)Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling Ki67 with ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse breast carcinoma. The section was incubated with ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab279653)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Ki67 with ab279653 at 1/1000 dilution (0.936 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat colon. The section was incubated with ab279653 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Anti-Ki67 antibody (ab279653)
