Anti-APG5L/ATG5 antibody (ab228668)
Key features and details
- Rabbit polyclonal to APG5L/ATG5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-APG5L/ATG5 antibody
See all APG5L/ATG5 primary antibodies -
Description
Rabbit polyclonal to APG5L/ATG5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human APG5L/ATG5 (internal sequence). The exact sequence is proprietary.
Database link: Q9H1Y0 -
Positive control
- ICC/IF HeLa cells and thapsigargin- or chloroquine-treated HepG2 cells. IHC-P: Mouse brain tissue. WB: NT2/D1 and PC-3 whole cell lysate; HeLa cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 50 µM chloroquine for 24 hours, whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 32 kDa15% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for APG5L/ATG5 (green) using ab228668 (1/1000 dilution) in ICC/IF. Cells mock (left panel) and treated with 50 μM chloroquine for 24 hours (right panel) were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Phalloidin, an F-actin marker (red).
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Paraffin-embedded mouse brain tissue stained for APG5L/ATG5 with ab228668 at 1/400 dilution in immunohistochemical analysis.
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All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution
Lane 1 : NT2/D1 (human embryonal carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 32 kDa12% SDS-PAGE
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for APG5L/ATG5 (green) using ab228668 (1/500 dilution) in ICC/IF. Cells were treated with 3 μM thapsigargin 16 hours (right panels) and mock (left panels) and were fixed in ice-cold methanol for 10 minutes, permeabilized with cooled acetone for 2 minutes.
Nuclear counterstain: Hoechst 33342 (blue).