Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)
![Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-255521-anti-apg5l-atg5-antibody-epr4797-western-blot.jpg "Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4797] to APG5L/ATG5
- Suitable for: Flow Cyt, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-APG5L/ATG5 antibody [EPR4797]
See all APG5L/ATG5 primary antibodies -
Description
Rabbit monoclonal [EPR4797] to APG5L/ATG5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- THP1, Raji, HeLa, HT1080 and Human fetal kidney lysates; Human breast carcinoma and Human kidney tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4797 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: APG5L/ATG5 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Jeg-3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109490 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109490 was shown to specifically react with APG5L/ATG5 when APG5L/ATG5 knockout samples were used. Wild-type and APG5L/ATG5 knockout samples were subjected to SDS-PAGE. ab109490 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-122369-anti-apg5l-atg5-antibody-epr4797-western-blot.jpg)
Western blot - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)All lanes : Anti-APG5L/ATG5 antibody [EPR4797] (ab109490) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
-
![Flow Cytometry - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-270689-anti-apg5l-atg5-antibody-epr4797-flow-cytometry.jpg)
Flow Cytometry - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling APG5L/ATG5 with purified ab109490 at 1/250 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
![Western blot - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-122368-anti-apg5l-atg5-antibody-epr4797-western-blot.jpg)
Western blot - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)Anti-APG5L/ATG5 antibody [EPR4797] (ab109490) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Predicted band size: 32 kDa
Upper band shows APG5L/ATG5 conjugated to ATG12. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-122367-anti-apg5l-atg5-antibody-epr4797-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)ab109490, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-122366-anti-apg5l-atg5-antibody-epr4797-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)ab109490, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human kidney by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)](https://www.abcam.com/ps/products/109/ab109490/Images/ab109490-7-benefits-of-recombinant-antibodies.png)
Anti-APG5L/ATG5 antibody [EPR4797] (ab109490)
