Formalin-fixed, paraffin-embedded human brain tissue stained for APG5L/ATG5 using ab238092 at 2 µg/ml in immunohistochemical analysis.

All lanes : Anti-APG5L/ATG5 antibody [ATG5/2101] (ab238092) at 2 µg/ml

Lane 1 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 2 : Human brain tissue lysate

Predicted band size: 32 kDa

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238092.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human endometrium tissue stained for APG5L/ATG5 using ab238092 at 2 µg/ml in immunohistochemical analysis.