Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

Key features and details

  • Mouse monoclonal [3B7CF2AC8] to ECH1
  • Suitable for: IHC-P, WB, ICC, Flow Cyt
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-ECH1 antibody [3B7CF2AC8]
    See all ECH1 primary antibodies

  • Description

    Mouse monoclonal [3B7CF2AC8] to ECH1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml

    Lane 1 : Human heart homogenate
    Lane 2 : Human liver homogenate
    Lane 3 : Rat heart homogenate
    Lane 4 : Cow heart homogenate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    All lanes : Goat-anti-Mouse HRP

    Predicted band size: 36 kDa

  • Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    Immunocytochemistry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
  • Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

    IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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