Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
![Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-393619-anti-ech1-antibody-epr15449b-c-terminal-western-blot-human-knockout-lysate.jpg "Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-ECH1 antibody [EPR15449(B)] - C-terminal
See all ECH1 primary antibodies -
Description
Rabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK293T, A549, U-2 OS, HeLa and Jurkat cells; Human fetal liver lysate, IHC-P: Human endometrium and kidney tissues. ICC/IF: MCF-7 cells. IP: Human fetal liver lysate. Flow Cyt: Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15449(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ECH1 knockout HEK293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1-4: Merged signal (red and green). Green - ab189255 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.
ab189255 Anti-ECH1 antibody [EPR15449(B)] was shown to specifically react with ECH1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266748 (knockout cell lysate ab257933) was used. Wild-type and ECH1 knockout samples were subjected to SDS-PAGE. ab189255 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224596-ab189255IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224595-ab189255IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224597-ab189255IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
-
![Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224598-ab189255WB.jpg)
Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
-
![Flow Cytometry - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224594-ab189255FC.jpg)
Flow Cytometry - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
-
![Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-224593-ab189255.jpg)
Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
-
![Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)](https://www.abcam.com/ps/products/189/ab189255/Images/ab189255-8-benefits-of-recombinant-antibodies.png)
Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
