Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ECH1 antibody [EPR15449(B)] - C-terminal
    See all ECH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15449(B)] to ECH1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, A549, U-2 OS, HeLa and Jurkat cells; Human fetal liver lysate, IHC-P: Human endometrium and kidney tissues. ICC/IF: MCF-7 cells. IP: Human fetal liver lysate. Flow Cyt: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ECH1 knockout HEK293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : U-2 OS cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab189255 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa. 

     ab189255 Anti-ECH1 antibody [EPR15449(B)] was shown to specifically react with ECH1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266748 (knockout cell lysate ab257933) was used.  Wild-type and ECH1 knockout samples were subjected to SDS-PAGE.  ab189255 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

  • Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Western blot - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Flow Cytometry - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Immunoprecipitation - Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

    Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)
    Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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