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Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)

Key features and details

  • Mouse monoclonal [4F3] to PI 3 Kinase catalytic subunit alpha/PIK3CA
  • Suitable for: WB, ELISA, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3]
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies

  • Description

    Mouse monoclonal [4F3] to PI 3 Kinase catalytic subunit alpha/PIK3CA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human PI 3 Kinase catalytic subunit alpha/PIK3CA.

  • Positive control

    • Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (AA: 881-1068), HEK293 cell lysate and PI 3 Kinase catalytic subunit alpha/PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819) at 1/500 dilution + Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (AA: 881-1068)

    Predicted band size: 47.4, 124 kDa

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    All lanes : Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PI 3 Kinase catalytic subunit alpha/PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 47.4, 124 kDa

  • ELISA - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    ELISA - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Example of standard curves obtained using ab126819. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Immunofluorescence analysis of HeLa cells using ab126819 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819)
    Flow cytometric analysis of Jurkat cells using ab126819 at 1/200 (green) and negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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