Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2E9] to beta Amyloid - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-beta Amyloid antibody [2E9] - BSA and Azide free
    See all beta Amyloid primary antibodies

  • Description

    Rat monoclonal [2E9] to beta Amyloid - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG, HEK-293 and HeLa whole cell lysate. Human brain and hippocampus tissue lysate. IHC-P: Alzheimer’s Disease cerebrum. IP: HeLa whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255772 is the carrier-free version of ab252816. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)

    Immunohistochemical analysis of paraffin-embedded Alzheimer’s Disease cerebrum tissue labeling beta Amyloid with ab252816
    at 0.583µg/ml, followed by ready to use secondary ab214882. Positive staining on Alzheimer’s Disease cerebrum tissue is observed (PMID: 1640495). The section was incubated with ab252816 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252816).

  • Western blot - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)
    Western blot - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)
    All lanes : Anti-beta Amyloid antibody [2E9] (ab252816) at 0.583 µg/ml

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : Human brain tissue lysate
    Lane 5 : Human hippocampus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 87 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 37 secs; Lanes 4-5: 5 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252816).

  • Immunoprecipitation - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)
    Immunoprecipitation - Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)

    Beta Amyloid was immunoprecipitated from 1mg of HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate with ab252816 at 19.433µg/ml. Western blot was performed from the immunoprecipitate using ab252816 at 1/1000 dilution. Goat-anti-Rat IgG for IP (HRP) (ab205720) was used at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab252816 IP in HeLa whole cell lysate.

    Lane 3: Rat monoclonal IgG instead of ab252816 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252816).

  • Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)
    Anti-beta Amyloid antibody [2E9] - BSA and Azide free (ab255772)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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