Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Amyloid antibody [DE2B4] (ab11132)

Key features and details

  • Mouse monoclonal [DE2B4] to beta Amyloid
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-beta Amyloid antibody [DE2B4]
    See all beta Amyloid primary antibodies

  • Description

    Mouse monoclonal [DE2B4] to beta Amyloid

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-17 of Human beta Amyloid.

  • Epitope

    ab11132 recognizes an epitope in the region 8-16 of the amyloid precursor protein.

  • Positive control

    • IHC-P: Alzheimer's disease human brain; Mouse brain tissue. IHC-Fr: Mouse brain cortex. WB: CHO-K1 cell lysate. ICC/IF: HEK cells.

  • General notes

    This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    DE2B4

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132)
    Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    ab11132 staining beta Amyloid in transgenic APPP23 mouse brain cortex by immunohistochemistry (frozen sections). Unfixed cryosections were fixed in 4% Formalin in PBS before treatment with 70% Formic acid to reveal antigenic sites. Fixed samples were permeabilized with 0.1% Tween 20, blocked with 1% BSA for 30 minutes at 1oC. Samples were incubated with primary antibody (1/200 in TBS/BSA/Tween 20/azide) for 2 hours. Anti-mouse IgG Alexa Fluor® 594 (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132) This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
    Immunofluorescence detection using Mouse monoclonal [DE2B4] to beta Amyloid (ab11132) on Human HEK cells. Cells were fixed using Methanol, no antigen retrieval step was necessary. Primary antibody ab11132 was incubated for 1hr at room temperature (1/400). The confocal image of transfected HEK cells shows A. anti-amyloid beta (ab11132), B. swAPP-GFP, C. Overlay. Significant colocalisation is seen as would be expected. (GFP is tagged to C-term of swAPP).

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