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Anti-beta Amyloid antibody [MOAB-2] (ab126649)

Key features and details

  • Mouse monoclonal [MOAB-2] to beta Amyloid
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-beta Amyloid antibody [MOAB-2]
    See all beta Amyloid primary antibodies

  • Description

    Mouse monoclonal [MOAB-2] to beta Amyloid

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human beta Amyloid. Recombinant oligomeric Aß42. Epitope maps to residues 1-4 of human Aß42 and Aß40 (DAEF).

  • Positive control

    • IHC-P: Human Hippocampus (Alzheimer's Disesase) tissue; Mouse brain tissue. WB: 5xFAD transgenic mouse (12 months) lysate.

  • General notes

    This antibody clone is manufactured by Abcam.

    The clone name for this antibody is also known as 6C3.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    MOAB-2

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] (ab126649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] (ab126649)
    IHC image of ab126649 staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] (ab126649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] (ab126649)
    IHC image of ab126649 staining in transgenic mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-beta Amyloid antibody [MOAB-2] (ab126649)
    Western blot - Anti-beta Amyloid antibody [MOAB-2] (ab126649) This image is courtesy of M.J. LaDu, Univ of IL, Chicago
    Lanes 1-2 : Anti-beta Amyloid antibody [MOAB-2] (ab126649) at 0.5 µg/ml
    Lanes 3-4 : Anti-beta Amyloid antibody [6E10] at 0.5 µg/ml
    Lanes 5-6 : Anti-beta Amyloid antibody [22C11] at 2 µg/ml

    Lanes 1 & 3 & 5 : Synthetic beta Amyloid 42 at 100 pmoles
    Lanes 2 & 4 & 6 : 5xFAD transgenic mouse (12 months) lysate at 25 µg

    Secondary
    All lanes : Anti-mouse IgG

    Developed using the ECL technique.

    Predicted band size: 87 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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