Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free (ab273481)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1B5] to Amyloid Precursor Protein - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Mouse monoclonal [1B5] to Amyloid Precursor Protein - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Alzheimer's disease cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273481 is the carrier-free version of ab273450. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273481 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free (ab273481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free (ab273481)

    This data was developed using ab273450 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human Alzheimer's disease cerebrum tissue labeling Amyloid protofibrils with ab273450 at 1/500  dilution followed by a ready to use secondary. Positive staining on human Alzheimer's disease cerebrum tissue is observed. The section was incubated with ab273450 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab190482 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is  LeicaDS9800 (Bond™ Polymer Refine Detection) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Not generated yet. (ab273481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Not generated yet. (ab273481)

    This data was developed using ab273450 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Amyloid protofibrils with ab273450 at 1/500 dilution followed by a ready to use secondary. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Negative control: Human cerebrum.

  • Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free (ab273481)
    Anti-Amyloid Precursor Protein antibody [1B5] - BSA and Azide free (ab273481)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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